Michael Wilde netted twice for Chester but that still wasn't enough, as Borough secured the win thanks to a last minute goal at the Exacta Stadium.
Neil Young received the Manager of the Month award for November prior to the game, and all inside the Stadium paid tribute to former players Paul Raynor and John Butler, and former Manager Harry McNally was remembered six years on from his passing.
Chester could and should have scored early on as first Wilde almost clinically backheeled into the goal in the opening minutes, before Kyle Wilson wasted a golden chance by blasting high over the bar.
Radcliffe then took the lead against the run of play as Chris Sanna conceded his first goal in disappointing fashion, as he spilled a Mark Connor shot straight into the path of Simon Kelly who made no mistake from close range to put the visitors one up on XX minutes.
Wilson missed another gilt edged chance when he ran on to a Bradley Barnes pass but could only find Radcliffe keeper Nick Culkin with a weak effort.
Chester managed to get back on level terms just six minutes before the half time break, as Wilson this time produced a great cross for Wilde to get his head on to and steer in the equalizer.
The second half saw a patchy performance by the Blues, the two week break in football didn't help matters, but just a quarter of an hour before the end, Alistair Brown scored arguably one of the greatest goals at the Exacta Stadium, as he let fly from 35 yards giving Sanna no chance as the ball flew into the top corner of the net.
Chester were leftr dumbstruck by the shot, but just a couple of minutes later they were back on level terms, as substitute Mark Connolly delivered a pin point corner for Wilde to glance home his 19th goal of the season.
The points were then up for grabs, but it seemed the Blues couldn't muster up a winner, and instead it was Borough who found themselves in acres of space as first Tom Brooks missed a sitter late on, before in the last minute of injury time another breakaway saw Mark Jones pass the ball past Sanna for a 3-2 win, and Chester's ten match unbeaten run was to come to an end.
Chester: Sanna, Stones, Jones, Ruffer, Williams, McCarthy (Connolly 65), Barnes, Field, Howard (Hopley 74), Wilde, Wilson. Subs: Booth, Beck, Graves.
Goals: Wilde 39, 76.
Radcliffe: Culkin, Brown, Kelly, S Connor, King (Brook 46), Sherlock, Sonko (Forrest 73), M Jones, Manning, M Connor (G Jones 65), Roscoe. Subs: Lourenco, Brobbin.
Goals: Kelly 22, Brown 74, Jones 90.
Referee: A Scregg (Liverpool)
Member since: 10th July 2012
Chester word of mouth specialist, promotes and markets the best businesses in Chester. Passionately supporting local businesses, organisations and events.